Chemical Reagents for Protein Modification, Fourth Edition by Adriane Y Togashi Affiliation: University of São Paulo, São

By Adriane Y Togashi Affiliation: University of São Paulo, São Paulo, Brazil;Fabiano R Cirano Affiliation: University of São Paulo, São Paulo, Brazil;Marcia M Marques Affiliation: University of São Paulo, São Paulo, Brazil;Francisco E Pustiglioni Affil

Aim: the purpose of the current examine used to be to evaluate the effect of the chemical features and roughness of titanium surfaces at the viability, proliferation and differentiation of osteoblast-like cells cultured in a medium supplemented with recombinant human bone morphogenetic protein-7 (rhBMP-7).
Material and strategies: Osteo-1 cells have been grown on titanium disks offering with the next surfaces: (1) machined, (2) coarse grit-blasted and acid-attacked (SLA) and (3) chemically converted SLA (SLAmod) within the absence or presence of 20 ng/ml rhBMP-7 in tradition medium. The viability and variety of osteo-1 cells have been evaluated after 24 h. Analyses of overall protein content material (TP) and alkaline phosphatase (AP) task at 7, 14 and 21 days, collagen content material at 7 and 21 days and mineralized matrix formation at 21 days have been performed.
Results: cellphone viability (P=0.5516), mobile quantity (P=0.3485), collagen content material (P=0.1165) and mineralized matrix formation (P=0.5319) weren't suffering from the various floor configurations or by means of the addition of rhBMP-7 to the medium. Osteo-1 cells cultured on SLA surfaces confirmed an important elevate in TP at 21 days. The ALPase/TP ratio (P=0.00001) was once laid low with remedy and time.
Conclusion: the implications recommend that the addition of rhBMP-7 to the tradition medium didn't exert any influence at the viability, proliferation or differentiation of osteoblast-like cells grown at the various surfaces proven. All titanium surfaces analyzed allowed the whole expression of the osteoblast phenotype akin to matrix mineralization by way of osteo-1 cells. Read more...

summary: Aim: the purpose of the current learn used to be to evaluate the impression of the chemical features and roughness of titanium surfaces at the viability, proliferation and differentiation of osteoblast-like cells cultured in a medium supplemented with recombinant human bone morphogenetic protein-7 (rhBMP-7).
Material and strategies: Osteo-1 cells have been grown on titanium disks proposing with the next surfaces: (1) machined, (2) coarse grit-blasted and acid-attacked (SLA) and (3) chemically changed SLA (SLAmod) within the absence or presence of 20 ng/ml rhBMP-7 in tradition medium. The viability and variety of osteo-1 cells have been evaluated after 24 h. Analyses of overall protein content material (TP) and alkaline phosphatase (AP) task at 7, 14 and 21 days, collagen content material at 7 and 21 days and mineralized matrix formation at 21 days have been performed.
Results: phone viability (P=0.5516), phone quantity (P=0.3485), collagen content material (P=0.1165) and mineralized matrix formation (P=0.5319) weren't laid low with different floor configurations or by way of the addition of rhBMP-7 to the medium. Osteo-1 cells cultured on SLA surfaces confirmed an important raise in TP at 21 days. The ALPase/TP ratio (P=0.00001) was once laid low with remedy and time.
Conclusion: the consequences recommend that the addition of rhBMP-7 to the tradition medium didn't exert any influence at the viability, proliferation or differentiation of osteoblast-like cells grown at the diverse surfaces verified. All titanium surfaces analyzed allowed the entire expression of the osteoblast phenotype resembling matrix mineralization through osteo-1 cells

Show description

Read Online or Download Chemical Reagents for Protein Modification, Fourth Edition PDF

Similar chemical books

Optical Guided-wave Chemical and Biosensors I

For the 1st time, distinctive scientists from key associations all over the world offer a complete method of optical sensing recommendations applying the phenomenon of guided wave propagation for chemical and biosensors. This contains either state-of the-art basics and cutting edge functions of those concepts.

Chemical Treatment

This monograph on chemical remedy is one in every of a chain of 8 on cutting edge website and waste remediation applied sciences which are the fruits of a multi association attempt concerning greater than a hundred specialists over a ­ yr interval. It presents the skilled, working towards expert assistance at the program of leading edge techniques thought of prepared for full-scale software.

Chemical Spectroscopy and Photochemistry in the Vacuum-Ultraviolet: Proceedings of the Advanced Study Institute, held under the Auspices of NATO and the Royal Society of Canada, August 5–17, 1973, Valmorin, Quebec, Canada

It truly is most likely secure to foretell that the way forward for chemistry is associated with the excited states of molecules and to different brief lived species, ions and loose radicals. Molecules have just one floor country yet many excited states. even if huge the scope of standard, flooring kingdom chemistry should be, above and past it lies the area of excited states, each having its personal chemis­ test.

Extra resources for Chemical Reagents for Protein Modification, Fourth Edition

Sample text

Chem. 388, 997–1006, 2007. 146. M. , A simple statistical method for discriminating outer membrane proteins with better accuracy, Bioinformatics 21, 961–968, 1995. 147. A. , The amino acid composition and morphology of some invertebrate and vertebrate collagens, Biochim. Biophys. Acta 34, 24–39, 1959. 148. , Olmo, N. , Biochemical and mechanical behavior of ostrich pericardium as a new biomaterial, Acta Biomater. 2, 213–219, 2006. 149. L. , Size distribution and amino acid chemistry of base-extractable proteins from Washington coast sediment, Biogeochemistry 75, 177–200, 2005.

Biophys. 122, 164–173, 1967. 145. , Abbas, M. , Selenium in chemistry and biochemistry in comparison to sulfur, Biol. Chem. 388, 997–1006, 2007. 146. M. , A simple statistical method for discriminating outer membrane proteins with better accuracy, Bioinformatics 21, 961–968, 1995. 147. A. , The amino acid composition and morphology of some invertebrate and vertebrate collagens, Biochim. Biophys. Acta 34, 24–39, 1959. 148. , Olmo, N. , Biochemical and mechanical behavior of ostrich pericardium as a new biomaterial, Acta Biomater.

222, 695–704, 1950. 10. , The therapeutic possibilities arising from the chemical modification of proteins, Proc. Natl. Acad. Sci. USA 53, 897–501, 1965. H. , Protein plastics from soy bean products. Action of handening or tanning agents on protein material, J. Ind. Eng. Chem. 30, 1236–1240, 1938. 12. , The cross linking of protein plastics, Br. Plastics Moulded Products Trader 23, 157–162, 1950. 13. , Chemical modification of proteins, US Patent 2562534, 1951. R. , Chemical Modification of Proteins, Holden-Day, San Francisco, CA, 1971.

Download PDF sample

Rated 4.08 of 5 – based on 32 votes