Cell Reproduction: In honor of Daniel Mazia by Ellen R. Dirksen

By Ellen R. Dirksen

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The above mentioned polypeptide, number 10, with an approximate molecular weight of 80,000 Daltons was unique in the sense that it was the only one that was newly synthesized when cells were activated to grow, that is when G^ was compared to the resting state GQ. i UJ Ksot-· r «SOIi P f ♦ ♦ £t ♦♦ ♦ tfistanc· tr·* *τψ* 2. M A X M. 4: Partial specific synthesis (PSS) values of nuclear envelopes in Go (A), mid G^ (B), late Gj/early S (C), late S/G2 (D) and metaphase/early G]_ (E) . The five arrows on each curve indicate the positions of the five bands with relatively high changes in biosynthesis during the cell cycle.

1963). Biol. 27,329. 32. M. (1973). 32, 91-101. 33. de Terra,N. (1975). Nature (London) 258, 300. CELL REPRODUCTION THE ROLE OF RNA PROCESSING IN GENE EXPRESSION Robert P. Perry The Institute for Cancer Research, Fox Chase Center, Philadelphia, Pennsylvania 19111 ABSTRACT This paper deals with some current ideas about the processing of eukaryotic messenger RNAs. The experimental bases for relating mRNA to heterogeneous nuclear RNA, for determining mRNA frequency distributions and for elucidating mRNA processing pathways are reviewed.

P. Gopinathan^, and T. Kunkel2 The Institute for Cancer Research The Fox Chase Cancer Center Philadelphia, Pennsylvania 19111 ABSTRACT The fidelity of DNA synthesis by purified DNA polymerases has been determined using synthetic polynucleotides and natural DNA templates. With poly d(A-T) as a template, the frequency of mis-incorporation of dGTP with different DNA polymerases varies from 1 in 3,000 to 1 in 100,000. Similar results have been obtained by measuring the reversion frequency of a single-base substitution on 0X174 DNA after copying by polymerases in vitro.

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