Biochemical Aspects of Prostaglandins and Thromboxanes: by Norman Kharasch

By Norman Kharasch

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Yamamoto o f Kyot o Universit y Facult y o f Engineerin g an d Mr . M. Hayash i an d Mr . H . Miyak e o f th e On o Centra l Researc h Institut e wh o synthesize d an d donate d thio l derivative s of prostaglandin . Thi s wor k ha s bee n supporte d i n par t b y a Grant-in-Ai d fo r Scientifi c Researc h fro m th e Ministr y of Education , Scienc e an d Cpltur e o f Japa n an d b y grant s fro m th e Japanes e Foundatio n o n Metabolis m an d Diseases , th e Fujiwar a Memoria l Foundation , an d th e Kana e Foundatio n o f th e New Medicin e Researc h Association .

56 , 529 (1976) . L. , Polsky-Cynkin , R. , an d Levine , L. , J . Biol . Chem. 251 , 77 6 (1976) . 38 Lawrenc e Levin e er al. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. L. , an d Levine , L. , J . Biol . Chem. 251 , 581 4 (1976) . L. , an d Levine , L. , Proc . Natl . Acad . Sci . US A 73, 173 0 (1976) . Polsky-Cynkin , R. L. , an d Levine , L. , J . Pharmacol . Exper . Therapeu . 197 , 56 7 (1976) . S. L. , an d Levine , L. , J . Biol . Chem. i n pres s (1977) . S. , an d Levine , L.

Treatmen t o f th e cell s wit h thes e drug s di d no t resul t i n a n increas e o f th e fre e arachidoni c aci d level s i n th e cells . a a a C. Effect s o f Cytochalasi n an d Colchicin e Cytochalasi n  inhibit s th e prostaglandi n productio n by MC5-5 cell s incubatin g i n th e presenc e o f serum ; th e exten t of inhibitio n i s a functio n o f concentratio n o f dru g (9) . At a concentratio n o f 5 yg/ml , 95 %o f th e prostaglandi n synthesi s b y th e cell s wa s inhibited ; a t a leve l o f 0.

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