By Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)
Confocal microscopy with laser scanning know-how yields in-vivo pictures of ocular and ocular adnexal surfaces which are so superb that they rival histology by way of quality.This exact atlas and textbook demonstrates basic in-vivo anatomy of the cornea, limbus and conjunctiva, quantifies a variety of mobile buildings utilizing cell-density calculations and establishes correlations among novel optical sections of assorted ailments of the ocular floor and medical findings. additionally, it helps the translation of novel high-magnification optical sections by means of evaluating corneal and conjunctival imprint cytology with in-vivo photographs and describes early inflammatory alterations in corneal grafts, in addition to corneal conjunctivalisation in limbal stem phone deficiency, corneal dystrophies or infections, flap interface and margin features after laser in-situ keratomileusis (LASIK). furthermore, it instructs the reader approximately diagnostic and healing follow-up suggestions and gives a short creation to functions in different fields comparable to dentistry and ear, nostril and throat surgery.
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Extra info for Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology
NaF also penetrates areas of microerosions and pathologically altered cells. The literature reveals discrepant views concerning the nature of the penetration process. Most authors subscribe to the view that the fluorescein fills the “footprint” spaces vacated by cells that have been lost. Others, however, assume that fluorescein fills the intercellular space. At present, only confocal slit-scanning microscopes and fluorophotometers are used to analyze this phenomenon. The HRA in combination with the RCM can be used for confocal laser scanning fluorescence microscopy of the microstructure of the corneal epithelium and tear film using contact and noncontact techniques (see Fig.
The inner mucin layer consists of glycoproteins. Its task is to make the epithelial surface hydrophobic and thus guarantee wettability. Fig. 1 Noncontact microscopy. Scanning laser reflex (arrow) on the cornea (argon laser/Heidelberg Retina Angiograph Classic) Fig. 2 Normal tear film 32 Chapter 5 Confocal Laser Scanning In Vivo Microscopy Replacing the contact system in the confocal laser scanning microscope with a dry objective lens, combined with an 80–90% absorption gray filter to decrease laser intensity (Fig.
A Slit-lamp microscopy. b Pentacam Scheimpflug camera (Oculus Optikgeräte, Wetzlar, Germany) c a e d b Fig. 37 Epidemic keratoconjunctivitis. Confocal image of the central cornea in epidemic keratoconjunctivitis; focal planes moved axially from epithelium to endothelium. a Intermediate epithelial layer with isolated hyperreflective round structures located between the cells. b Basal cell layer with hyperreflective dendritic network. c–d Transition from basal cell layer to nerve plexus layer with dendritic cell structures, some of which appear to be spread out between the nerve fibers.