By Thomas M. Bell (Auth.)
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The cells then grew out from the tissue into the surrounding clot. 2 ' 3 In 1928 the Maitland cultures followed. 5 The use of the enzyme trypsin6'7 in preparing monolayer cultures set the seal and today the bulk of all virological investigations is carried out using one or other of the above techniques. In this chapter the methods of preparing tissue cultures will be outlined and reference will be made to their present-day uses. Although plant, insect and cold-blooded animal tissues can be cultivated8»9'10 only techniques for the growth of mammalian and avian tissue will be mentioned.
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40. 45 Porterfield, J. S. (1962) Advances in Virus Research, 9, 127. Hirst, G. K. (1941) Science, 94, 22. , Hare, R. (1941) Canad. Pub. Health J. 32, 530. Horsfall, F. L. (1954) / . Exper. Med. 100, 135. , Fox, J. P. (1957) Proc. Soc. Exp. Biol. Med. 96, 788. Lerner, A. ML, Cherry, J. , Finland, M. (1963) Virology, 19, 58. , Burnet, F. M. (1957) Virology, 4, 305. Pereira, H. , Figueiredo, M. V. T. (1962) Virology, 18, 1. Burnet, F. , Stone, J. (1947) Aust. J. Exp. Biol. Med. Sei. 25, 227. Hirst, G.